INTERNAL TRANSCRIBED SPACER (ITS), AN IDEAL DNA BARCODE FOR SPECIES DISCRIMINATION IN CRAWFURDIA WALL. (GENTIANACEAE)
نویسندگان
چکیده
منابع مشابه
Internal Transcribed Spacer (its), an Ideal Dna Barcode for Species Discrimination in Crawfurdia Wall. (gentianaceae)
BACKGROUND DNA barcoding is a technique used to identify species based on species-specific differences in short regions of their DNA. It is widely used in species discrimination of medicinal plants and traditional medicines. MATERIALS AND METHODS In the present study, four potential DNA barcodes, namely rbcL, matK, trnH-psbA and ITS (nuclear ribosomal internal transcribed spacer) were adopted...
متن کاملNuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi.
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variabl...
متن کاملLimits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi.
In PNAS, the work by Schoch et al. (1) proposed nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as the sole universal barcode for fungi. The work by Schoch et al. (1) stated that “the proposal will satisfy most fungal biologists but not all” (1). I am among those biologists who are not so satisfied and not really surprised either, because ITS has been the most widely used mark...
متن کاملEvaluating the Ribosomal Internal Transcribed Spacer (ITS) as a Candidate Dinoflagellate Barcode Marker
BACKGROUND DNA barcoding offers an efficient way to determine species identification and to measure biodiversity. For dinoflagellates, an ancient alveolate group of about 2000 described extant species, DNA barcoding studies have revealed large amounts of unrecognized species diversity, most of which is not represented in culture collections. To date, two mitochondrial gene markers, Cytochrome O...
متن کاملInternal transcribed spacer 2 barcode: a good tool for identifying Acanthopanacis cortex
Acanthopanacis cortex has been used in clinical applications for a long time. Considering some historical and geographical factors, Acanthopanacis cortex is easily confused with other herbs in medicine markets, thereby causing potential safety issues. In this study, we used the internal transcribed spacer 2 (ITS2) barcode to identify 69 samples belonging to six species, including Acanthopanacis...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: African Journal of Traditional, Complementary and Alternative medicines
سال: 2016
ISSN: 0189-6016,2505-0044
DOI: 10.21010/ajtcam.v13i6.15